Modules - Nucleus Documentation

Overview¶

A Module is a useful biochemical formulation, often incorporating a genetically encoded component, that performs a particular function. Modules Specifications answer the questions 1) “What is it?” and 2) “What should I expect when I implement it?”.

Validation key: ★★★ frequently used, ★★ validated (cells or in vitro), ★ preliminary / DevNote only

PURExpress¶

Modules validated in NEB PURExpress.

Module Class	Specification	Validation
Membrane (Base)	POPC/Chol	★★★
Detector	tetR-aTc	★★
Emitter	IV-HSL	★★
Control	ClpXP	★★
Energy	PPK	★
Membrane Pore	α-Hemolysin	★
	Cx43	★

Nucleus Cytosol¶

Modules validated in Nucleus Cytosol.

Module Class	Specification	Validation
Cytosol (Base)	Cytosol	★★★
Membrane (Base)	POPC/Chol	★★★
Reporter	deGFP	★★★

Contributing a Module¶

Modules specifications are derived from DevNotes that follow the adhere to the Module Contribution Guidelines.