Overview¶
Processes represent the core protocols. They tell you how to transform physical materials into Base Cytosol and Cell. Modules extend the functionality of Base Cytosol and Cell.
Base Cell Processes¶
The Base Cell is formed by encapsulating Base Cytosol (see below) in a liposome.
Protocol: Inverted Emulsion, a second protocol for giant unilamellar vesicles
Base Cytosol Processes¶
Base Cytosol is a molecular system with a defined set of components including T7 RNA Polymerase, ribosomes, and tRNA capable of transcription and translation. Base Cytosol builds on the PURE system, and is optimized for integration and extension.
Build a Base Cell — the lipids, an outer solution and an inner solution of Base Cytosol, in order, through to finished Base Cells.
Make Base Cytosol Components¶
Quality Control Processes¶
Cascade Processes¶
Cascade Processes make the parts of a two-liposome cascade and read the result. The gel is set by Embedding: Thermal Setting.
Embedding Processes¶
Embedding holds a sample in a gel. The route depends on what sets the gel.
- Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K., & Ueda, T. (2001). Cell-free translation reconstituted with purified components. Nature Biotechnology, 19(8), 751–755. 10.1038/90802