Overview¶
Processes represent the core protocols. They tell you how to transform physical materials into Base Cytosol and Cell. Modules extend the functionality of Base Cytosol and Cell.
Base Cell Processes¶
The Base Cell is formed by encapsulating Base Cytosol (see below) in a liposome.
Schematic of Base Cell Processes
Figure 1:Flow chart for building Base Cell and its modification through the addition of Modules.
Base Cytosol Processes¶
Base Cytosol is a molecular system with a defined set of components including T7 RNA Polymerase, ribosomes, and tRNA capable of transcription and translation. Base Cytosol builds on the PURE system, and is optimized for integration and extension.
Schematic of Base Cytosol Processes
Figure 2:Flow chart for building Base Cytosol and its modification through the addition of Modules.
Make Base Cytosol Components¶
Quality Control Processes¶
- Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K., & Ueda, T. (2001). Cell-free translation reconstituted with purified components. Nature Biotechnology, 19(8), 751–755. 10.1038/90802