Overview¶
Making PURE requires making Protein Mix, which contains the thirty-six (36) proteins in the PURE system. If you want to purify each protein individually, you can follow our 36-pot protocol. However, if you want working protein mix with less time and effort, you can co-express and co-purify all 36 proteins at once in OnePot. This protocol shows you how. This protocol has been established to grow, purify, and characterize OnePot PURE proteins following Lavickova’s OnePot PURE protocol with a few deviations.
Important Information
Please read this section carefully. It contains important notes, resources, and safety information. Not all information included here is included in the lab-ready protocol.
Notes
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Prerequisite Documentation
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Hazardous Materials
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Critical Materials
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Genetically Encoded Components
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Composition
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References
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Protocol¶
Under Construction
Downloads¶
- Grasemann, L., Lavickova, B., Elizondo-Cantú, M. C., & Maerkl, S. J. (2021). OnePot PURE Cell-Free System. Journal of Visualized Experiments, 172. 10.3791/62625