Skip to article frontmatterSkip to article content
Site not loading correctly?

This may be due to an incorrect BASE_URL configuration. See the MyST Documentation for reference.

Overview

Making PURE requires making Protein Mix, which contains the thirty-six (36) proteins in the PURE system. If you want to purify each protein individually, you can follow our 36-pot protocol. However, if you want working protein mix with less time and effort, you can co-express and co-purify all 36 proteins at once in OnePot. This protocol shows you how. This protocol has been established to grow, purify, and characterize OnePot PURE proteins following Lavickova’s OnePot PURE protocol with a few deviations.

References
  1. Grasemann, L., Lavickova, B., Elizondo-Cantú, M. C., & Maerkl, S. J. (2021). OnePot PURE Cell-Free System. Journal of Visualized Experiments, 172. 10.3791/62625