Overview¶
This protocol describes the Process for making the thirty-six component proteins of the Base Cytosol. It can be broken down into 5 sub-processes:
Prepare buffers and gravity columns.
Grow cultures of E. coli expression strains for each protein.
Lyse each culture and purify target proteins by Ni2+ affinity column.
Measure the concentration and purity of each protein.
Combine proteins into a final Protein Mix.
Important Information
Please read this section carefully. It contains important notes, resources, and safety information. Not all information included here is included in the lab-ready protocol.
Notes
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Prerequisite Documentation
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Hazardous Materials
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Critical Materials
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Genetically Encoded Components
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Composition
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References
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Protocol¶
Under Construction