Overview¶
This page is a single, distribution-wide index of all materials referenced in this Distribution. Use it when authoring or reviewing a contribution to reuse an existing part number and vendor rather than introducing a near-duplicate.
Note: this table is automatically generated.
The table below is generated from bom-<process> tables on the Process pages by scripts/build-materials-reference.py. It is rebuilt at every deploy and is never committed, so it cannot drift from the source pages (the same single-source-of-truth model as the lab-ready protocol PDFs — see the lab-ready pipeline notes in CLAUDE.md). To regenerate it locally, run python3 scripts/build-materials-reference.py before jupyter book start or myst build. To add or correct an entry, edit the Materials in the appropriate Process page, not this page.
Distribution-wide materials reference
| Name | Category | Product | Manufacturer | Part # | Price | Storage | Link | Used in |
|---|---|---|---|---|---|---|---|---|
| BL21(DE3) Competent Cells | Biologic | BL21(DE3) Competent E. coli | NEB | C2527H | $252 | -85 °C to -75 °C | link | make-1pot |
| Chloroform | Chemical | Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer | Sigma-Aldrich | 366927 | $94.30 | RT (flammables cabinet) | link | assemble-base-cell |
| Glucose | Chemical | D-(+)-Glucose, 99% | Thermo Scientific | A16828-36 | $41.65 | RT | link | assemble-base-cell, dye-liposomes |
| HEPES pH 7.6 | Chemical | HEPES, Free Acid | Fisher BioReagents | NC1584172 | $85.90 | RT | link | dye-liposomes |
| Mineral oil | Chemical | Mineral oil, mixed weight | Thermo Scientific | AC415080010 | $53.40 | RT | link | assemble-base-cell, dye-liposomes |
| Butyl HIC columns (5 mL) | Columns | HiTrap Butyl HP Column | Cytiva | 28-4110-05 | $679.00 | 4 °C to 30 °C | link | make-ribosomes |
| Columns | Columns | Empty Disposable PD-10 Columns | Cytiva | 17043501 | $258.30 | 4 °C to 30 °C | link | make-1pot, purify-proteins-grav-column |
| 18-well imaging slide | Consumable | µ-Slide 18 Well glass bottom | ibidi | 80826 | $178.00 | RT | link | dye-liposomes |
| 384-well glass bottom plate | Consumable | 384 well glass bottom plate, 1.5 cover glass (20/case) | Cellvis | P384-1.5H-N | $423.00 | RT | link | assemble-base-cell |
| 96-well optical plate | Consumable | Microplate, 96 well, PS, U-bottom, clear | Greiner | 650101 | $156.14 | 4 °C to 30 °C | link | pierce660 |
| Glass vials | Consumable | Vials, screw top, graduated, solid cap, preassembled, volume 4 mL, clear glass vial, thread 13-425, O.D. x H 15 mm x 45 mm, PTFE liner | Supelco | 27506-U | $157.41 | RT | link | assemble-base-cell |
| 0.22 µm syringe filters | Consumables | PES Syringe Filter, 0.22µm, 30mm, Sterile | CELLTREAT | 229747 | $84 | 4 °C to 30 °C | link | make-ribosomes, prep-consumables |
| 0.22 µm vacuum filters | Consumables | Corning® 500 mL Vacuum Filter, 0.22 µm PES Membrane, Sterile | Corning | 431097 | $187.14 | 4 °C to 30 °C | link | prep-consumables |
| 0.45 µm syringe filters | Consumables | PES Syringe Filter, 0.45μm, 30mm, Sterile | CELLTREAT Scientific Products | 229749 | $84 | 4 °C to 30 °C | link | lysis-sonication |
| 1 L centrifuge bottles | Consumables | Thermo Scientific™ # Fiberlite 1000mL Bottles | Thermo Scientific | 010-1491 | $326.84 | 4 °C to 30 °C | link | grow-bacteria, make-ribosomes, make-trna |
| 10 mL Luer Lock Syringes | Consumables | Syringes with BD Luer-Lok® Tip, BD Medical, Syringe with Luer-Lok® Tip, Volume=10 mL | BD Industrial/Difco | 550-80620-PK | $102.40 | 4 °C to 30 °C | link | lysis-sonication, prep-consumables |
| 100 kDa centrifugal filter | Consumables | Amicon® Ultra Centrifugal Filter, 100 kDa MWCO | EMD Millipore | UFC910008 | $141.00 | 4 °C to 30 °C | link | make-ribosomes |
| 15 mL centrifuge tubes | Consumables | Corning® 15 mL PP Centrifuge Tubes, Rack Packed with CentriStar™ Cap, Sterile, 50/Rack, 500/Case | Corning | 430790 | $230.44 | 4 °C to 30 °C | link | lysis-sonication, make-ribosomes, make-trna, prep-consumables, purify-proteins-grav-column |
| 50 mL centrifuge tubes | Consumables | Corning® 50 mL Polypropylene Centrifuge Tubes, Sterile, Racked, CentriStar™ Cap | Corning | 430828 | $436.88 | 4 °C to 30 °C | link | make-trna |
| 96-well Polypropylene DeepWell Plates | Consumables | Nunc™ 96-Well Polypropylene DeepWell™ Sample Processing & Storage Plates with Shared-Wall Technology | Nunc | 260251 | $379.61 | 4 °C to 30 °C | link | make-1pot |
| Amicon Ultra 0.5 mL - 3 kDa | Consumables | Amicon® Ultra Centrifugal Filter, 3 kDa MWCO | Millipore Sigma | UFC5003 | $63 | 4 °C to 30 °C | link | buff-ex-spin-filters, make-1pot, make-trna |
| Amicon Ultra 15 mL - 3 kDa | Consumables | Amicon® Ultra Centrifugal Filter, 3 kDa MWCO | Millipore Sigma | UFC9003 | $125 | 4 °C to 30 °C | link | buff-ex-spin-filters, make-1pot |
| Breathe-Easy sealing membrane | Consumables | Breathe-Easy® sealing membrane | Sigma-Aldrich | Z380059-1PAK | $96.50 | 4 °C to 30 °C | link | make-1pot |
| Culture tubes | Consumables | 14mL Culture Tube and Dual Cap, PP, Sterile | CELLTREAT | 230439 | $190.00 | 4 °C to 30 °C | link | grow-bacteria, make-ribosomes, make-trna |
| Polycarbonate ultracentrifuge bottles (70 mL) | Consumables | 70 mL Polycarbonate Bottle Assembly, 38 x 102mm - 6Pk | Beckman Coulter | 355622 | $687.60 | 4 °C to 30 °C | link | make-ribosomes |
| Rapid-Flow Sterile Single Use Vacuum Filter Units | Consumables | Nalgene™ Rapid-Flow™ Sterile Disposable Bottle Top Filters (PES, 0.22 µm) | Thermo Scientific | 596-3320 | $105.97 | 4 °C to 30 °C | link | make-1pot |
| SealPlate film | Consumables | SealPlate® film | Excel Scientific | Z369659-100EA | $72.20 | 4 °C to 30 °C | link | make-1pot |
| Slide-A-Lyzer G3 Dialysis Cassettes (3.5 kDa) | Consumables | Slide-A-Lyzer™ G3 Dialysis Cassettes, 3.5K MWCO | Thermo Scientific | A52966 | $128.70 | 4 °C to 30 °C | link | make-trna |
| TBE-Urea 10% gel | Consumables | 10% Mini-PROTEAN | Bio-Rad | 4566033 | $41.00 | 2 °C to 8 °C | link | make-trna |
| Tris-Glycine 4–20% gels | Consumables | 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 50 µL | BioRad | 4561094 | $153.00 | 1 °C to 4 °C | link | make-ribosomes, protein-gel |
| -20 °C Freezer | Equipment | TSX Series High-Performance -20 °C Manual Defrost Freezers | Thermo Scientific | TSX2320FA | unlisted | 4 °C to 30 °C | link | grow-bacteria |
| -80 °C Freezer | Equipment | TSX Series Ultra-Low Freezers | Thermo Scientific | TSX60086A | unlisted | 4 °C to 30 °C | link | grow-bacteria |
| Bench centrifuge | Equipment | Sorvall X4R Pro-MD, IVD Certified | Sorvall | 75009521 | $18,270.00 | 4 °C to 30 °C | link | grow-bacteria |
| Gel Imager | Equipment | ChemiDoc™ Imaging System | BioRad | 12003153 | unlisted | 4 °C to 30 °C | link | protein-gel |
| Gel Power Supply | Equipment | PowerPac™ Basic Power Supply | BioRad | 1645050 | $530.00 | 4 °C to 30 °C | link | protein-gel |
| Gel Tank | Equipment | Criterion™ Cell | BioRad | 1656001 | $852.00 | 4 °C to 30 °C | link | protein-gel |
| Glass serological pipette 10 mL | Equipment | PYREX® 10 mL Disposable Glass Serological Pipets, TD, Individually Wrapped, Sterile, Plugged | Corning | 7077-10N | $690.49 | RT | link | assemble-base-cell, make-trna |
| Glass syringe 250 µL | Equipment | Hamilton glass syringe | Hamilton | 14-815-238 | $150.15 | RT | link | assemble-base-cell, dye-liposomes |
| Lab Jack | Equipment | Fisherbrand™ Lab Jacks (15cmx15cm) | Fisher Scientific | 14-673-51 | $484 | 4 °C to 30 °C | link | lysis-sonication |
| Microvolume spectrophotometer | Equipment | DeNovix DS-11+ Spectrophotometer | DeNovix | DS-11+ | unlisted | 4 °C to 30 °C | link | grow-bacteria |
| Plate Reader | Equipment | BioTek Cytation 5 Cell Imaging Multimode Reader | Agilent | CYT5MFAWSN | Quote required | 4 °C to 30 °C | link | pierce660 |
| Sonicator | Equipment | Q700 Sonicator | QSonica | Q700A-110 | $5500 | 4 °C to 30 °C | link | lysis-sonication |
| Sonicator Probe | Equipment | Standard Probe, Replaceable Tip, 1/2" (12.7mm) diameter | QSonica | 4220 | $670 | 4 °C to 30 °C | link | lysis-sonication |
| Sound Enclosure | Equipment | Sound Enclosure | QSonica | 432B2 | $995 | 4 °C to 30 °C | link | lysis-sonication |
| Thermocycler | Equipment | SimpliAmp™ Thermal Cycler | Applied Biosystems | A24811 | $6,580.00 | 4 °C to 30 °C | link | protein-gel |
| 2 L baffled Erlenmeyer flasks | Flasks | PYREX® 2L Delong Shaker Erlenmeyer Flask with Baffles | Corning | 4444-2L | $96.44 | 4 °C to 30 °C | link | grow-bacteria, make-ribosomes, make-trna |
| Cholesterol | Lipid | Cholesterol (plant) | Avanti Research | A80100 | $261.00 | -20 °C | link | assemble-base-cell |
| Liss-Rhod PE | Lipid | 18:0 Liss Rhod PE 1 mg/mL | Avanti Lipids | A81179 | $273.47 | -20 °C | link | assemble-base-cell, dye-liposomes |
| POPC | Lipid | 16:0-18:1 PC 25 mg/mL | Avanti Lipids | A80557 | $435.00 | -20 °C | link | assemble-base-cell, dye-liposomes |
| IPTG | Media | Isopropyl β-D-thiogalactoside (IPTG), Powder, ≥99% (TLC), ≤0.1% Dioxane | Sigma-Aldrich | I6758-1G | $89.90 | -25 °C to -15 °C | link | grow-bacteria, make-1pot, prep-consumables |
| Kanamycin | Media | Kanamycin sulfate, BioReagent, ≥750 µg/mg, Suitable for cell culture, Powder | Sigma-Aldrich | K1377-1G | $47.70 | 4 °C to 30 °C | link | grow-bacteria, prep-consumables |
| LB | Media | Luria Broth (Miller’s LB Broth), Non-Sterile, pH 6.8-7.2, Molecular Biology Grade, Powder | Sigma-Aldrich | L3522-1KG | $221 | 4 °C to 30 °C | link | grow-bacteria, make-ribosomes, make-trna, prep-consumables |
| rATP | NTP | Adenosine 5′-triphosphate disodium salt hydrate, BioXtra, ≥99% (HPLC) | Sigma-Aldrich | A7699-1G | $125.00 | -25 °C to -15 °C | link | make-small-molecule-mix |
| rCTP | NTP | Cytidine 5′-triphosphate disodium salt, ≥95% | Sigma-Aldrich | C1506-100MG | $102.00 | -25 °C to -15 °C | link | make-small-molecule-mix |
| rGTP | NTP | Guanosine 5′-triphosphate sodium salt hydrate, ≥95% (HPLC), powder | Sigma-Aldrich | G8877-100MG | $155 | -25 °C to -15 °C | link | make-small-molecule-mix |
| rUTP | NTP | Uridine 5′-triphosphate trisodium salt hydrate, Type IV, ≥93.0% (HPLC) | Sigma-Aldrich | U6750-100MG | $58.20 | -25 °C to -15 °C | link | make-small-molecule-mix |
| 10x Tris/Glycine SDS running buffer | Reagent | # 10x Tris/Glycine/SDS | BioRad | 1610732 | $41.00 | 4 °C to 30 °C | link | protein-gel |
| Amino acids | Reagent | L-Amino acids, analytical standard | Sigma-Aldrich | LAA21-1KT | $558 | 1 °C to 4 °C | link | make-1pot, make-amino-acid-mix |
| BME | Reagent | 2-Mercaptoethanol | Sigma-Aldrich | M6250-100ML | $65.50 | 4 °C to 30 °C | link | make-small-molecule-mix |
| BSA Protein Standard | Reagent | Pierce™ Bovine Serum Albumin Standard Ampules, 2 mg/mL | Thermo Scientific | 23209 | $81.65 | 4 °C to 30 °C | link | pierce660 |
| Creatine phosphate | Reagent | Sodium creatine phosphate dibasic tetrahydrate, ≥98.0%(NT) | Sigma-Aldrich | 27920-1G | $102 | -25 °C to -15 °C | link | make-1pot, make-small-molecule-mix |
| Folinic acid | Reagent | Folinic acid calcium salt hydrate | Sigma-Aldrich | F7878-100MG | $108 | 4 °C to 30 °C | link | make-1pot, make-small-molecule-mix |
| HPTS | Reagent | HPTS (Pyranine) | Thermo Scientific | L11252-06 | $92.60 | RT | link | dye-liposomes |
| Optiprep | Reagent | OptiPrep™ | STEMCELL Technologies | 07820 | $289.00 | RT | link | assemble-base-cell, dye-liposomes |
| Pierce660 Reagent | Reagent | Pierce™ 660nm Protein Assay Reagent | Thermo Scientific | 22660 | $176.65 | 4 °C to 30 °C | link | pierce660 |
| Protein Ladder | Reagent | Precision Plus Protein™ Unstained Protein Standards | BioRad | 1610363EDU | $138.00 | -25 °C to -15 °C | link | protein-gel |
| PURExpress Kit | Reagent | PURExpress® In Vitro Protein Synthesis Kit | NEB | E6800L | $2774.00 | -85 °C to -75 °C | link | make-1pot |
| Rapid protein stain | Reagent | Pageblue™ Protein Staining Solution | Thermo Scientific | 24620 | $153.65 | 4 °C to 30 °C | link | protein-gel |
| RNase Inhibitor, Murine | Reagent | RNase Inhibitor, Murine | NEB | M0314S | $87.00 | -25 °C to -15 °C | link | make-1pot |
| Spermidine | Reagent | Spermidine, ≥99% (GC) | Sigma-Aldrich | S2626-5G | $176 | -25 °C to -15 °C | link | make-1pot, make-small-molecule-mix |
| TCEP | Reagent | Thermo Scientific, Bond-Breaker® Tris[2-Carboxyethyl]phosphine Neutral Solution (TCEP), Application=Water-Soluble Reducing Agent, Molecular Weight=286.65, Size=5 mL | Thermo Scientific | 77720 | $175.65 | 4 °C to 30 °C | link | lysis-sonication, make-1pot, make-ribosomes, make-small-molecule-mix, prep-consumables, purify-proteins-grav-column |
| Ni-His Resin | Resin | NEBExpress Ni Resin | New England Biolabs | S1428S | $358.00 | 1 °C to 4 °C | link | make-1pot, purify-proteins-grav-column |
| Ammonium chloride | Salts | Ammonium chloride, ACS reagent, ≥99.5% | Sigma-Aldrich | 213330-500G | $73.50 | 4 °C to 30 °C | link | lysis-sonication, make-1pot, make-ribosomes, prep-consumables, purify-proteins-grav-column |
| Ammonium Sulfate | Salts | Ammonium sulfate,BioXtra, ≥99.0% | Sigma-Aldrich | A2939-1KG | $188.00 | 4 °C to 30 °C | link | make-ribosomes |
| EDTA | Salts | Ethylenediaminetetraacetic acid, BioUltra, ≥99.0% (KT) | Sigma-Aldrich | 03609-250G | $139.00 | 4 °C to 30 °C | link | make-1pot |
| HEPES | Salts | HEPES, Crystalline Powder, ≥99.5% (titration), Poly bottle | Sigma-Aldrich | H3375-500G | $431 | 4 °C to 30 °C | link | lysis-sonication, make-1pot, make-ribosomes, make-small-molecule-mix, prep-consumables, purify-proteins-grav-column |
| Hydrochloric acid (1M) | Salts | Hydrochloric acid 1 N, Reagent Grade, 500 mL | VWR | E447-500L | $72.46 | 4 °C to 30 °C | link | make-small-molecule-mix, prep-consumables, purify-proteins-grav-column |
| Imidazole | Salts | ReagentPlus® Imidazole, Crystalline, 99% | Sigma-Aldrich | I202-500G | $120 | 4 °C to 30 °C | link | make-1pot, prep-consumables, purify-proteins-grav-column |
| Magnesium acetate | Salts | Magnesium acetate tetrahydrate | Sigma-Aldrich | M0631-100G | $34.80 | 4 °C to 30 °C | link | make-1pot, make-ribosomes, make-small-molecule-mix, make-trna |
| Magnesium chloride | Salts | Magnesium chloride hexahydrate, BioXtra, ≥99.0% | Sigma-Aldrich | M2670-500G | $115.00 | 4 °C to 30 °C | link | make-1pot |
| Potassium chloride | Salts | Potassium Chloride, ACS reagent, 99.0-100.5% | Sigma-Aldrich | P3911-1KG | $146.00 | 4 °C to 30 °C | link | make-1pot, make-ribosomes, prep-consumables, purify-proteins-grav-column |
| Potassium glutamate | Salts | L-Glutamic acid potassium monohydrate | Sigma-Aldrich | G1501-100G | $74.80 | 4 °C to 30 °C | link | make-1pot, make-small-molecule-mix |
| Potassium hydroxide | Salts | Potassium hydroxide - ACS reagent, ≥85%, pellets | Sigma-Aldrich | 221473-1KG | $99.90 | 4 °C to 30 °C | link | lysis-sonication, make-1pot, make-ribosomes, make-small-molecule-mix, prep-consumables, purify-proteins-grav-column |
| Sodium Acetate | Salts | Sodium acetate trihydrate,ACS reagent, ≥99% | Sigma-Aldrich | 236500-500G | $87.90 | 4 °C to 30 °C | link | make-trna |
| Sodium Hydroxide (pellets) | Salts | Sodium hydroxide, ACS Reagent Grade, Pellets, ≥97.0%, Poly bottle | Sigma-Aldrich | 221465-1KG | $137.00 | 4 °C to 30 °C | link | make-ribosomes |
| Magnesium Chloride hexahydrate | Salts (for Buffers) | BioXtra Magnesium chloride, Hexahydrate, Powder or Crystals, ≥99.0% | Sigma-Aldrich | M2670-100G | $50.50 | 4 °C to 30 °C | link | lysis-sonication, prep-consumables, purify-proteins-grav-column |
| Sodium Chloride | Salts (for Buffers) | Sodium Chloride, Redi-Dri™, anhydrous, free-flowing, ACS, ≥99% | Sigma-Aldrich | 746398-1KG | $133.00 | 4 °C to 30 °C | link | lysis-sonication, make-ribosomes, make-trna, prep-consumables, purify-proteins-grav-column |
| A19 E. coli | Strains | A19 (RNase I mutant) | CGSC | 5997 | $130.00 | -80 °C | link | make-ribosomes, make-trna |
| 2x TBE-Urea sample buffer | Supplements | Invitrogen novex TBE-Urea Sample Buffer (2x) | Thermo Scientific | LC6876 | $75.60 | 2 °C to 8 °C | link | make-trna |
| Acetic Acid (glacial) | Supplements | Acetic Acid, Glacial (Certified ACS), Fisher Chemical | Fisher Scientific | A38-212 | $458 | 4 °C to 30 °C | link | make-ribosomes, make-trna |
| Acid Phenol (pH 4.5) | Supplements | Invitrogen ambion Acid Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) | Fisher Scientific | AM9722 | $313.65 | 2 °C to 8 °C | link | make-trna |
| Ethanol | Supplements | Ethanol, HPLC/Spectrophotometric Grade, Liquid, ≥99.5%, Glass bottle, 200 Proof | Sigma-Aldrich | 459828-4L | $493.00 | 4 °C to 30 °C | link | make-ribosomes, make-trna |
| Glycerol | Supplements | Glycerol, Molecular Biology Grade, Liquid, ≥99.0% | Sigma-Aldrich | G5516-1L | $157.00 | 4 °C to 30 °C | link | make-1pot, prep-consumables |
| Isopropanol | Supplements | BioReagent 2-Propanol, Molecular Biology Grade, Liquid, ≥99.5% | Sigma-Aldrich | I9516-1L | $142.00 | 4 °C to 30 °C | link | make-trna |
| Laemmli Buffer (6x) | Supplements | Laemmli SDS-Sample Buffer (6x, Reducing) | Boston BioProducts | BP-111R | $42.00 | 4 °C to 30 °C | link | make-ribosomes, protein-gel |
| NEB low range ssRNA ladder | Supplements | Low Range ssRNA Ladder - 100 gel lanes | New England Biolabs | N0364S | $87.00 | -25 °C to -15 °C | link | make-trna |
| Sucrose | Supplements | Sucrose, bioultra, for molecular biology, ≥99.5% (HPLC) | Sigma-Aldrich | 84097-1KG | $170.00 | 4 °C to 30 °C | link | make-ribosomes |
| SYBR-Green stain | Supplements | Invitrogen sybr Green I Nucleic Acid Gel Stain 10,000x concentrate in DMSO | Fisher Scientific | S7567 | $791.70 | -25 °C to -15 °C | link | make-trna |
| cOmplete Protease Inhibitor | Supplements (for Buffers) | cOmplete™ Protease Inhibitor Cocktail, EDTA-Free, Tablets | Roche | 11873580001 | $472 | 1 °C to 4 °C | link | lysis-sonication, prep-consumables |
| Lysozyme | Supplements (for Buffers) | Lysozyme from chicken egg white, protein ≥90%, ≥40 000 units/mg protein, lyophilized powder | Sigma-Aldrich | L6876-1G | $76.90 | -25 °C to -15 °C | link | lysis-sonication, prep-consumables |