Overview¶
The Base Membrane specifies a set of components that are used to make the lipid bilayers defining the Base Cell. The membrane is a composed of (POPC), cholesterol, and fluorescent Lissamine rhodamine PE. The Base Membrane represents a sane default for making synthetic cells. The Base Cell is made by encapsulating Base Cytosol in Base Membrane following the Process Assemble Base Cell.
Schematic
Designs
Schematic of a POPC/Chol liposome.
None
Reference Composition¶
| Component | Target Percentage (%) | Molecular Weight (g/mol) | Stock concentration (mg/mL) | Volume to add (uL) |
|---|---|---|---|---|
| POPC | 70 | 760.076 | 25 | 162.17 |
| Cholesterol | 29.95 | 386.654 | 50 | 17.65 |
| Liss-Rhod PE | 0.05 | 1301.71 | 1 | 4.96 |
Expected Behavior¶
The behavior of Base Cell composed of Base Membrane is characterized using the deGFP Reporter Module.
Protocols¶
Protocols for assembling Base Cell and making its components from scratch are described in the Process Assembling Base Cell.