Overview¶
The Base Membrane specifies a phospholipid bilayer composed of POPC, cholesterol, and a fluorescent lipid label. Liss-Rhod PE or 18:1 Cy5-PC can serve as the label. The Base Membrane is the recommended default membrane for making synthetic cells by using Encapsulation: Phase Transfer.
Schematic of a POPC/Chol liposome.
Reference Composition¶
| Component | Target (mmol/mol) | Molecular Weight (g/mol) | Stock concentration (mg/mL) | Volume to add (µL) |
|---|---|---|---|---|
| POPC | 699.3 | 760.076 | 25 | 162.01 |
| Cholesterol | 299.7 | 386.654 | 50 | 17.66 |
| Fluorescent lipid label | 1 | See the dye menu | See the dye menu | See the dye menu |
Dye menu. One dye fills the fluorescent lipid label row, at the same 1 mmol/mol.
| Dye | Catalog | Molecular Weight (g/mol) | Stock concentration (mg/mL) | Volume to add (µL) |
|---|---|---|---|---|
| Liss-Rhod PE | Avanti A81150 | 1301.71 | 1 | 9.92 |
| 18:1 Cy5-PC | Avanti A85483 | 1316.26 | 1 | 10.03 |
Recipes of the Base Membrane. The default is the first row.
| Recipe | POPC (mmol/mol) | Cholesterol (mmol/mol) | Fluorescent lipid label (mmol/mol) | Used for |
|---|---|---|---|---|
| Default | 699.3 | 299.7 | 1 | Base Cell |
| 9:1 | 899.1 | 99.9 | 1 | GUVs by inverted emulsion transfer, with the tetR-aTc Detector |
| Pure POPC | 999 | 0 | 1 | Cells with the EsaR detector or the LuxR detector, including the LuxR demo |
Editor: link the Implementations that use the pure POPC recipe once their pages exist.
Expected Behavior¶
Cells¶
The Base Membrane Module is expected to hold an inner solution (the “cytosol”) separate from an outer solution.
A recipe with a different cholesterol fraction is expected to differ in bilayer permeability.
Editor: cite a source for the cholesterol and permeability claim.
Processes¶
Encapsulation: Phase Transfer — prepares synthetic cells by encapsulating an inner solution in a membrane.
Credits¶
Developed by b.next, with dye menu and recipes from Mary Kelly (Chicago Node, Kamat Lab).
Editor: name the authors of the membrane comparison DevNote, “London Exchange Meeting: Liposome Protocol Survey”, which lists its authors only as “London Exchange Meeting Participants”, or confirm it is not a source of the recipes.
Supported by¶
DevNotes that support this specification include:
