Overview¶
The Base Membrane specifies a phospholipid bilayer composed of (POPC), cholesterol, and fluorescent Lissamine Rhodamine PE (Liss-Rhod PE). The Base Membrane is our recommended default membrane for making synthetic cells by using Encapsulation: Phase Transfer.
Schematic
Schematic of a POPC/Chol liposome.
Reference Composition¶
| Component | Target Percentage (%) | Molecular Weight (g/mol) | Stock concentration (mg/mL) | Volume to add (µL) |
|---|---|---|---|---|
| POPC | 70 | 760.076 | 25 | 162.17 |
| Cholesterol | 29.95 | 386.654 | 50 | 17.65 |
| Liss-Rhod PE | 0.05 | 1301.71 | 1 | 4.96 |
Expected Behavior¶
The behavior of Base Membrane is characterized using the deGFP Reporter Module in Base Cell.
Protocols¶
Protocols for assembling Base Cell and making its components from scratch are described in the Process Encapsulation: Phase Transfer.