Overview¶
Gel: LGA is a physical hydrogel of low gelling agarose (LGA) in an outer solution. The network sets on cooling, at about 24 °C to 28 °C, so the stock is held at 45 °C until it is mixed with a sample. It is set by Embedding: Thermal Setting.
Reference Composition¶
Outer solution of Gel: LGA.
| Component | Type |
|---|---|
| Outer solution | Solution |
Polymer of Gel: LGA.
| Component | Stock concentration | Final concentration |
|---|---|---|
| Low gelling agarose | 28 mg/mL (2.8 % w/v) | 7 mg/mL (0.7 % w/v) |
The final concentration is for a stock diluted 1:1 with outer solution and then mixed 1:1 with the sample.
Expected Behavior¶
Gel: LGA is expected to set when the mixture cools to its gel point, about 24 °C to 28 °C. It can set at room temperature, so the stock is kept warm until the mixture is dispensed.
Requirements¶
Requires an outer solution to dissolve into. For liposomes, use the outer solution used to prepare the liposomes.
Requires that a sample tolerate mixing at 45 °C.
Processes¶
Embedding: Thermal Setting prepares the stock and sets the gel.
Materials¶
Materials of Gel: LGA.
| Name | Category | Product | Manufacturer | Part # | Price | Storage | Link |
|---|---|---|---|---|---|---|---|
| Low gelling agarose | Polymer | Low gelling agarose | Sigma-Aldrich | A9414-5G | — | Room temperature | Sigma-Aldrich A9414 |
Constituent Modules¶
Outer solution — the solution the polymer dissolves into
Low gelling agarose — the polymer
Credits¶
Developed by Ojaswita Pant (Northwestern University, DevCells Chicago Node, Truby Lab) and Manuel Bibrowski (Imperial College London, DevCells London Node, Elani Lab).
Supported by¶
DevNotes that support this specification include:
