Component	Stock Concentration	Final Concentration in Reaction
PMix	15 mg/mL	1.8 mg/mL
SMix	4X	1X
Ribosomes	10 $\mu$ M	1.8 $\mu$ M
tRNA	varies	3.5 mg/mL
pOpen-deGFP	varies	3 nM
Magnesium acetate	200 mM	varies

Component	Input concentration	Unit	Final concentration	Unit	Volume for one reaction (µL)
SMix	3.33	×	1	×	12
PMix	15	mg/mL	1.80	mg/mL	4.8
Ribosomes	10	µM	1.8	µM	7.2
`pOpen-deGFP` DNA	124	nM	3	nM	0.95
tRNA	35	mg/ml	3.5	mg/ml	4
Magnesium acetate	200	mM	8	mM	1.6
Optiprep	1.32	mg/µL	0.043	mg/µL	1.33
RNase Inhibitor	40000	U/mL	2000	U/mL	2
Water					6.12
Total volume (µL)					40

Component	Target Percentage (%)	Molecular Weight (g/mol)	Stock concentration (mg/mL)	Volume to add (µL)
POPC	70	760.076	25	162.17
Cholesterol	29.95	386.654	50	17.65
Liss-Rhod PE	0.05	1301.71	1	4.96

Reporter: deGFP

Overview¶