Overview¶
Gel: ULGA is a physical hydrogel of ultra-low gelling agarose (ULGA) in an outer solution. The network sets on cooling, at about 8 °C to 15 °C. It is set by Embedding: Thermal Setting.
Reference Composition¶
Outer solution of Gel: ULGA.
| Component | Type |
|---|---|
| Outer solution | Solution |
Polymer of Gel: ULGA.
| Component | Stock concentration | Final concentration |
|---|---|---|
| Ultra-low gelling agarose | 10 mg/mL (1.0 % w/v) | 5 mg/mL (0.5 % w/v) |
The final concentration is for a stock mixed 1:1 with the sample.
Expected Behavior¶
Gel: ULGA has a gel point of about 8 °C to 15 °C. The mixture sets at this temperature. The stock is easy to pipette. The ULGA stock is held at 55 °C until use.
Requirements¶
Requires an outer solution to dissolve into. For liposomes, use the outer solution used to prepare the liposomes.
Requires that a sample tolerate the temperature at which it is mixed with the stock.
Processes¶
Embedding: Thermal Setting prepares the stock and sets the gel.
Materials¶
Materials of Gel: ULGA.
| Name | Category | Product | Manufacturer | Part # | Price | Storage | Link |
|---|---|---|---|---|---|---|---|
| Ultra-low gelling agarose | Polymer | Ultra-low gelling agarose | Sigma-Aldrich | A5030 | — | Room temperature | Sigma-Aldrich A5030 |
Constituent Modules¶
Outer solution — the solution the polymer dissolves into
Ultra-low gelling agarose — the polymer
Credits¶
Developed by Ojaswita Pant (Northwestern University, DevCells Chicago Node, Truby Lab) and Manuel Bibrowski (Imperial College London, DevCells London Node, Elani Lab).
Supported by¶
DevNotes that support this specification include:
